ORIGINAL ARTICLE

Aktivitas hepatoprotektif Moringa oleifera pada tikus yang diinduksi streptozotocin

Dini Cynthia Dewi Tanuwijaya, Ali Santosa , Suryono Suryono

Dini Cynthia Dewi Tanuwijaya
Fakultas Kedokteran Universitas Jember, Jember, Indonesia

Ali Santosa
Departemen/KSM Ilmu Penyakit Dalam, Fakultas Kedokteran, Universitas Jember-RSD dr. Soebandi Jember, Jember, Indonesia. Email: [email protected]

Suryono Suryono
Departemen/KSM Ilmu Penyakit Jantung dan Pembuluh Darah, Fakultas Kedokteran, Universitas Jember-RSD dr. Soebandi Jember, Jember, Indonesia
Online First: April 01, 2021 | Cite this Article
Tanuwijaya, D., Santosa, A., Suryono, S. 2021. Aktivitas hepatoprotektif Moringa oleifera pada tikus yang diinduksi streptozotocin. Medicina 52(1): 27-29. DOI:10.15562/medicina.v52i1.1042


Introduction: Recent lifestyle changes are driving a shift from infectious-diseases to non-communicable diseases. Diabetes mellitus is a disease with a high morbidity, which caused by its ability to trigger some complications to other organs, such as liver. This study aims to determine the hepatoprotective activity of Moringa oleifera leaf extract on SGOT and SGPT levels of diabetes-induced rats.

Method: The study design was a post-test only control group design. A total of 28 adult Wistar rats weighing 200-250 g were divided into 7 groups. Animals in group A were orally treated daily with 2 ml of normal saline (NS), while B-G were rendered diabetic by single intraperitoneal injections of 45 mg/kg/BW of streptozotocin. They were then treated with 2 ml of NS, as well as 62,5, 125, 250, 500, and 1.000 mg/kg/BW of MoLE, respectively for 28 days. Then, rats were taken blood samples to be checked for serum SGOT and SGPT levels.

Results: From the examination results, serum SGOT and SGPT levels in the MoLE-given groups were lower than the non-MoLE-given group.

Conclusion: We conclude that Moringa oleifera leaf extract hepatoprotective activity can reduce the SGOT and SGPT levels on diabetes-induced rats.

 

Latar Belakang: Perubahan gaya hidup yang marak terjadi akhir-akhir ini mendorong terjadinya pergeseran pola penyakit dari penyakit infeksi menjadi penyakit tidak menular. Diabetes melitus menjadi salah satu penyakit dengan angka morbiditas yang cukup tinggi, yang disebabkan karena kemampuannya untuk mampu memicu komplikasi ke organ lain, seperti hepar. Penelitian ini berfungsi untuk mengetahui aktivitas hepatoprotektif ekstrak daun Moringa oleifera pada tikus yang diinduksi diabetes.

Metode: Penelitian ini menggunakan post-test only control group design. Sebanyak 28 ekor tikus wistar dengan berat 200-250 g dibagi ke dalam 7 kelompok. Tikus kelompok A diberi 2 ml normal saline (NS) sementara kelompok B-G diinjeksi dengan streptozotocin 45 mg/kgBB. Selanjutnya, kelompok B diberi 2 ml NS dan kelompok C-G diberi ekstrak daun kelor dosis 62,5, 125, 250, 500, dan 1.000 mg/kgBB selama 28 hari. Selanjutnya, masing-masing tikus diambil sampel darahnya dan dilakukan pemeriksaan kadar SGOT dan SGPT serum.

Hasil: Dari hasil pemeriksaan, kadar SGOT dan SGPT serum pada kelompok yang diberi ekstrak daun kelor lebih rendah (p < 0,05) dibanding kelompok yang tidak diberi ekstrak.

Kesimpulan: Aktivitas hepatoprotektif ekstrak daun kelor mampu menurunkan kadar SGOT dan SGPT pada tikus yang diinduksi diabetes.

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